Detection of Aflatoxin B1, B2, G1 and G2 in Rhizosphere and Rhizosplane of (zea Mays) Maize Plants Using Liquid Chromatography – Mass Spectrometry

An easy method for the determination of aflatoxins B1, B2, G1, and G2 in rhizosphere and rhizosplane of zea mays by LC coupled to mass spectrometry had been developed. Aflatoxins B1, B2, G1 and G2 in maize plants by LC coupled to mass spectrometry had been developed. Aflatoxins were extracted with a mixture of methanol and water and then it was purified by solid – phase clean – up using a polymeric sorbent. The eluted extracts were injected into the chromatographic system using a reverse – phase C18 short column with an isocratic mobile phase composed of methanol-water (30:70). A single quadruple mass spectrometry using an electro spray ionization source operating in the positive mode was used to detect aflatoxins. The method permits the detection of Aflatoxins with a detection limit of 0.01-14.90μg/g.